Luke C, Frenzel P. 2011. Potential of pmoA amplicon pyrosequencing for methanotroph diversity studies. Applied and Environmental Microbiology 77: 6305-6309.
Year Published:
2011
Abstract:
We analyzed the potential of pmoA amplicon pyrosequencing compared to that of Sanger sequencing with paddy soils as a model environment. We defined operational taxonomic unit (OTU) cutoff values of 7% and 18%, reflecting methanotrophic species and major phylogenetic pmoA lineages, respectively. Major lineages were already well covered by clone libraries; nevertheless, pyrosequencing provided a higher level of diversity at the species level.
Article ID:
1281