San Francisquito Creek (San Mateo County, CA) is home to a wide range of organisms, including several native fish species and an abundance of aquatic invertebrates. Because the creek passes through a diverse range of conditions, it is an ideal place to measure the impacts of human disturbance on aquatic organisms. This study aims to determine the relationship between human activity and aquatic invertebrate diversity and abundance. Samples were taken from five sites along the creek, three sites within a biological preserve, and two sites in more developed areas. Organisms were identified to order and compared across the five locations for diversity, abundance, and order richness. The data show that, in some cases, there does appear to be a relationship between human influence and abundance; however, a larger study is probably necessary to reveal a significant pattern in diversity and abundance in relation to human activity.