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Alvarez-Pérez S, De Vega C, Vanoirbeek K, Tsuji K, Jacquemyn H, Fukami T, Michiels C, Lievens B  (2023) Phylogenomic analysis of the genus Rosenbergiella and description of Rosenbergiella gaditana sp. nov., Rosenbergiella metrosideri sp. nov., Rosenbergiella epipactidis subsp. epipactidis subsp. nov., Rosenbergiella epipactidis subsp. californiensis subsp. nov., Rosenbergiella epipactidis subsp. japonicus subsp. nov., Rosenbergiella nectarea subsp. nectarea subsp. nov. and Rosenbergiella nectarea subsp. apis subsp. nov., isolated from floral nectar and insects. International Journal of Systematic and Evolutionary Microbiology 73(3):005777. https://doi.org/10.1099/ijsem.0.005777

Year Published: 2023
Abstract: 

The genus  is one of the most frequent bacterial inhabitants of flowers and a usual member of the insect microbiota worldwide. To date, there is only one publicly available  genome, corresponding to the type strain of  (8N4T), which precludes a detailed analysis of intra-genus phylogenetic relationships. In this study, we obtained draft genomes of the type strains of the other  species validly published to date ( and ) and 23 additional isolates of flower and insect origin. Isolate S61T, retrieved from the nectar of an  sp. flower collected in southern Spain, displayed low average nucleotide identity (ANI) and  DNA–DNA hybridization (isDDH) values when compared with other  members (≤86.5 and ≤29.8 %, respectively). Similarly, isolate JB07T, which was obtained from the floral nectar of  plants in Hawaii (USA) had ≤95.7 % ANI and ≤64.1 % isDDH with other  isolates. Therefore, our results support the description of two new  species for which we propose the names  sp. nov. (type strain: S61T=NCCB 100789T=DSM 111181T) and  sp. nov. (JB07T=NCCB 100888T=LMG 32616T). Additionally, some  and  isolates showed isDDH values<79 % with other conspecific isolates, which suggests that these species include subspecies for which we propose the names  subsp.  subsp. nov. (S256T=CECT 8502T=LMG 27956T),  subsp.  subsp. nov. (FR72T=NCCB 100898T=LMG 32786T),  subsp.  subsp. nov. (K24T=NCCB 100924T=LMG 32785T),  subsp.  subsp. nov. (8N4T = DSM 24150T = LMG 26121T) and  subsp.  subsp. nov. (B1AT=NCCB 100810T= DSM 111763T), respectively. Finally, we present the first phylogenomic analysis of the genus  and update the formal description of the species  and  based on new genomic and phenotypic information.  [link to publication]

Article Title: 
Phylogenomic analysis of the genus Rosenbergiella and description of Rosenbergiella gaditana sp. nov., Rosenbergiella metrosideri sp. nov., Rosenbergiella epipactidis subsp. epipactidis subsp. nov., Rosenbergiella epipactidis subsp. californiensis subsp.